Electronic microscopy (EM) uses electrons instead of photons for light and fluorescence microscopy. This technique provides higher resolution but is not compatible with live-imaging. In Transmission EM (TEM), a thin-section of a tissue fixed and stained with heavy metals to give contrast by preventing the electron beam from going across the section. In Scanning EM (SEM), the sample is dehydrated and coated with a layer of metal. The electron beam interacts with the metal atoms on the samples, and the emitted secondary electrons are detected by the microscope, giving information about the surface of the sample.